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KMID : 0644120150280020001
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Journal of Oriental Gynecology
2015 Volume.28 No. 2 p.1 ~ p.14
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Effects of Taraxaci Herba on Cell Death in Breast Cancer Cells
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Seo Kook-Jang
Park Kyung-Mi
Joh Sung-Hee
Yang Seung-Jeong
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Abstract
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Objectives: This study was designed to investigate the effects of Taraxaci Herba (TH) on cell death in breast cancer cells.
Methods: In this experiment, the effects of TH on proliferation rates, cell morphology and growth pattern, intracellular reactive oxygen species (ROS) production. In addition, the effects on nuclear condensation, fragmentation and formation of acidic vesicular organelles (AVO) in MCF-7 cells were also investigated. Finally, autophagy related with protein was observed by using western blot method.
Results: TH inhibited proliferation of MCF-7 cells, TH elevated intracellular ROS levels significantly. Treatment with TH did not affect nuclear morphologies such as condensation or fragmentation. On the other hand, TH treatment effectively induced AVO. Finally, one of autophagy related with protein, Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, LC3) level was elevated by treatment with TH.
Conclusions: These data indicate that TH is able to be used for patient with breast cancer and mechanisms are involved in autophagy through ROS generation.
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KEYWORD
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Taraxaci Herba, breast cancer cell, autophagy
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